Library Sizes ranged between 16,297,641 and 26,510,005 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Transcriptome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Transcriptome
Universal Adapter Content
Total overrepresented sequences for each library
## Overrepresented_sequences missing from RNASeq-11_R2.fastq.gz
## Overrepresented_sequences missing from RNASeq-12_R2.fastq.gz
## Overrepresented_sequences missing from RNASeq-20_R2.fastq.gz
## Overrepresented_sequences missing from RNASeq-23_R2.fastq.gz
## Overrepresented_sequences missing from RNASeq-24_R2.fastq.gz
## `summarise()` has grouped output by 'Sequence'. You can override using the `.groups` argument.
R version 4.1.0 (2021-05-18)
Platform: x86_64-w64-mingw32/x64 (64-bit)
locale: LC_COLLATE=English_Australia.1252, LC_CTYPE=English_Australia.1252, LC_MONETARY=English_Australia.1252, LC_NUMERIC=C and LC_TIME=English_Australia.1252
attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base
other attached packages: pander(v.0.6.4), DT(v.0.18), scales(v.1.1.1), readr(v.1.4.0), dplyr(v.1.0.6), stringr(v.1.4.0), magrittr(v.2.0.1), ngsReports(v.1.8.1), tibble(v.3.1.2), ggplot2(v.3.3.5) and BiocGenerics(v.0.38.0)
loaded via a namespace (and not attached): Rcpp(v.1.0.7), lubridate(v.1.7.10), lattice(v.0.20-44), tidyr(v.1.1.3), Biostrings(v.2.60.1), zoo(v.1.8-9), assertthat(v.0.2.1), digest(v.0.6.27), utf8(v.1.2.1), plyr(v.1.8.6), R6(v.2.5.0), GenomeInfoDb(v.1.28.1), stats4(v.4.1.0), evaluate(v.0.14), httr(v.1.4.2), pillar(v.1.6.1), zlibbioc(v.1.38.0), rlang(v.0.4.11), lazyeval(v.0.2.2), data.table(v.1.14.0), jquerylib(v.0.1.4), S4Vectors(v.0.30.0), rmarkdown(v.2.9), labeling(v.0.4.2), htmlwidgets(v.1.5.3), RCurl(v.1.98-1.3), munsell(v.0.5.0), compiler(v.4.1.0), xfun(v.0.23), pkgconfig(v.2.0.3), htmltools(v.0.5.1.1), tidyselect(v.1.1.1), GenomeInfoDbData(v.1.2.6), IRanges(v.2.26.0), fansi(v.0.5.0), viridisLite(v.0.4.0), crayon(v.1.4.1), withr(v.2.4.2), MASS(v.7.3-54), bitops(v.1.0-7), grid(v.4.1.0), jsonlite(v.1.7.2), gtable(v.0.3.0), lifecycle(v.1.0.0), DBI(v.1.1.1), stringi(v.1.6.2), farver(v.2.1.0), reshape2(v.1.4.4), XVector(v.0.32.0), bslib(v.0.2.5.1), ellipsis(v.0.3.2), ggdendro(v.0.1.22), generics(v.0.1.0), vctrs(v.0.3.8), RColorBrewer(v.1.1-2), tools(v.4.1.0), forcats(v.0.5.1), glue(v.1.4.2), purrr(v.0.3.4), crosstalk(v.1.1.1), hms(v.1.1.0), yaml(v.2.2.1), colorspace(v.2.0-2), plotly(v.4.9.4.1), knitr(v.1.33) and sass(v.0.4.0)